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All Journal JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia Journal of Mathematical and Fundamental Sciences EduChemia (Jurnal Kimia dan Pendidikan) Jurnal Ilmiah Arena Tekstil JURNAL SELULOSA Proceeding Biology Education Conference Indonesian Journal of Chemical Studies (Indones. J. Chem. Stud.)
ZEILY NURACHMAN
Kelompok Keahlian Biokimia FMIPA – ITB Jl Ganesha No. 10, Bandung
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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Journal : Jurnal Natur Indonesia

 1 
Isolation and Characterization of Phytase from Chicken Manure Bacteria Irawan, Mega Pratiwi; Nurachman, Zeily
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.527 KB) | DOI: 10.31258/jnat.15.2.99-105

Abstract

Cereals in animal feed contain anti-nutrients of phytic acid that has capability of chelating proteins and cations. Phytasecan be employed to reduce phytic acid through hydrolyzing phytic acid into free phosphate group and lower derivate ofinositol phosphate. The aim of the study was to isolate and characterize phytase obtained from chicken manure bacteria.The study included the screening of phytase-producing bacteria from chicken manure, the homology analysis of bacterium,and the determination of phytase activity. Phytase activity was measured from concentration of free phosphate. The resultsshowed that one phytase-producing isolate obtained from chicken manure grew in the medium containing 5% rice branextract at 37°C for 5 d. Based on a phylogeny is tree analysis of the genes related to 16S rRNA, the isolate was identified asAcinetobacter sp. TZ1. The extracellular phytase expressed by Acinetobacter sp. TZ1 exhibited optimum reactions at pH5 and 50°C. The enzyme showed activity of 64,6 nmol mL–1min–1 and specific activity of 236 nmol min–1mg–1. Relativemolecular mass of phytase TZ1 was ~35 kDa. Phytase obtained is potential to improve animal feed quality by hydrolyzingphytic acid.
Co-Authors Anceu Murniati Arie Hardian Buchari Buchari Cica Kasipah, Cica Dera Triani Elva Stiawan Enny Ratnaningsih IHSANAWATI IHSANAWATI Kelly Sinaga Mega Pratiwi Irawan, Mega Pratiwi Nobuo Tanaka Rina Masriani Sherly Sapulete Sinta Rismayani, Sinta Suryo Gandasasmita Sutomo Sutomo Tairo Oshima Vina Juliana Anggraeni Yanti Rachmayanti